Objective: To investigate in vitro transdermal absorption properties of Yinhu Ganmao spongia. Method: The content of chlorogenic acid was determined by HPLC and GC determination of linalool content

release was detected by Franz diffusion cell method with 10% ethanol as receiving liquid

transdermal receiving liquid at different time points was collected

and calculated cumulative permeation amount and ratio. Result: Regression equations of cumulative permeation amount of chlorogenic acid and linalool were Q=7.143 8t+8.229 4(r=0.996 1)

Q=125.375t-143.705(r=0.989 8)

respectively.J of them were 7.144

125.375 μg·cm-2·h-1

respectively. Conclusion: Chlorogenic acid in Yinhu Ganmao spongia continuous permeated uniformly within 24 h

but linalool almost permeated compeletly in 12 h;This method was simple and rapid

it was a ideal method to comprehensive evaluate in vitro transdermal performances of Yinhu Ganmao spongia.