Objective: To establish a method for determination of the dissolution of Breviscapine Pill. Method: Agilent TC-C18 column (4.6 mm×250 mm

5 μm) was used to separate the target components. The mobile phase was composed of methanol-tetrahydrofuran-0.1% phosphate solution (14:14:72). The flow rate was 0.8 mL·min-1 and the detection wavelength was set at 335 nm. Phosphate BS (pH 6.8) was used as solvent. Paddle method was used with rotation rate at 50 r·min-1. Scutellarin content was assayed by HPLC and the release percentage was then calculated. Result: The linear range of scutellarin was 20.8-416 μg·L-1 (r=0.999 9) with an average recovery of 99.92% (RSD 0.43%). The accumulation release percentage in 20 minutes was not lower than 70% of the content of scutellarin. Conclusion: The differences of the accumulation release percentage were shown in identical batch and the different batches samples. The method is repeatable for the quality control of Breviscapine Pill.