Objective: To set up the method for simultaneous determination of four active compounds in Chimonanthus nitens by HPLC. Method: The chromatography separation was performed on the Elite hypersil ODS2 (4.6 mm×250 mm

5 μm) with gradient elution. The mixture of acetonitrile and water as the mobile phases at a flow rate of 1.0 mL·min-1 was used

the detective wavelength was set at 344 nm and the column temperature was kept at 40℃. Result: The calibration curve was linear in the range of 5.665-56.65 mg·L-1 for scopoletin (r=0.999 9)

the average recovery was 97.06% and RSD was 1.62%. The calibration curve was linear in the range of 2.431 2-24.312 mg·L-1 for isofraxidin (r=0.999 9)

the average recovery was 102.86% and RSD was 0.93%. The standard curve presented a linear range from 2.444-24.44 mg·L-1 for scoparone (r=0.999 9)

the average recovery was 102.18% and RSD was 1.81%. The standard curve presented a linear range from 9.012-90.12 mg·L-1 for rutin (r=0.999 2)

the average recovery was 104.44% and RSD was 4.2%. Conclusion: The method is available with a good reproducibility

sensitive and simple and could be used to control the quality of C. nitens.