Objective: To investigate relativity between the content of rutin and traditional specification grade of Farfarae Flos. Method: The content of rutin from Farfarae Flos was performed by HPLC on Dikma Diamonsil C18 column (4.6 mm×250 mm

5 μm) with acetonitrile-1%acetic acid (20:80) as mobile phase and detection wavelength at 254 nm.HPLC fingerprint and similarity analysis of different specifications of Farfarae Flos was performed on Dikma Diamonsil C18 column (4.6 mm×250 mm

5 μm) with acetonitrile-0.1%H3PO4 gradient elution and detection wavelength of 240 nm. Result: Average content of rutin in superior grade was slightly higher than gradeless samples with little difference;Similarity of fingerprint of Farfarae Flos from different grades

habitats and batches was more than 0.85.Average values of twelve relative peak area of superior samples were larger than that of gradeless samples. Conclusion: Quantification method of rutin and fingerprint analysis was feasible and reproductive.Traditional classification of Farfarae Flos had a certain rationality

but the content of rutin had little correlation with traditional classification

so to classificate Farfarae Flos with the content of rutin as main index was unreasonable.