Objective: To develop an ultra performance liquid chromatography tandem mass (UPLC-MS-MS) method for determination of notoginsenoside R1

gensenoside Rg1

and gensenoside Rb1 in Chinese formula KLW. Method: The Chinese formula KLW is extracted by ultrasonic and centrifugal

then filtered before test. The ultra performance liquid chromatographic separation was performed on a Waters ACQUITY BEH C18 column (2.1 mm×50 mm

1.7 μm) by gradient elution with methanol-water as the mobile phase at a flow rate of 0.4 mL·min-1. The analysis of 3 compounds was performed under ESI multiple reaction monitoring (MRM) mode

and the quantification was conducted by external calibration method. The monitoring ions for notoginsenoside R1 were m/z 1 007.2/423.3 m/z; gensenoside Rg1 were 875.2/423.5 m/z; gensenoside Rb1 were 1 183.3/487.3 m/z. Result: The method showed a good linearity in the range of 0.05-2.0 mg·L-1

with the correlation coefficient above 0.999.The average recoveries (n=6) of notoginsenoside R1

ginsenoside Rg1 and ginsenoside Rb1 were 97.7%

96.3%

97.1% respectively. Conclusion: This method is simple

rapid

accurate and sensitive

can also provide a reliable method for clinical research and can be used for the quality control of Chinese formula KLW.