Objective: To establish a method for determining the content of naringin and naringenin in rat liver microsomes by UPLC

and preliminary investigation in vitro metabolism of two ingredients at different times points. Method: Determination was performed on UPLC chromatographic system

ACQUITY BEH C18 column (3.0 mm×100 mm

1.7 μm) with a mobile phase of methanol-0.1% acetic acid and gradient elution at a flow rate of 0.5 mL·min-1

detection wavelength was 283

289 nm

column temperature was 30℃

with quercetin as an internal standard

injection volume was 3 μm. Result: Naringin

naringenin and internal standard quercetin had good separation degree and no endogenous interference.The linear range of naringin and naringenin were 3.814-38.143(r=0.999 2)

3.586-35.857 mg·L-1(r=0.999 6)

respectively.Method recoveries of naringin ranged from 95.43% to 97.95% and absolute recoveries ranged from 97.33% to 98.18%.Method recoveries of naringenin ranged from 95.27% to 99.31% and absolute recoveries ranged from 97.71% to 99.73%.I phase metabolism of naringenin in in vitro liver microsomes was more significant than naringin. Conclusion: This method was rapid

sensitive and accurate

it could be used for determination of naringin and naringenin in rat liver microsomes.