Effect of Xinfukang Oral Liquid on CK-MM mRNA and CK-MM Protein Expression Level in Rat’s Heart Failure Model after Myocardial Infarction

HU Yuan-hui; DU Bai; FANG Su-ping; MA Tie-ming; FENG Xuan-chao; JIANG Bei; YANG Zheng

doi:10.11653/syfj2013160206

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Effect of Xinfukang Oral Liquid on CK-MM mRNA and CK-MM Protein Expression Level in Rat’s Heart Failure Model after Myocardial Infarction

更新时间：2024-01-04

- Effect of Xinfukang Oral Liquid on CK-MM mRNA and CK-MM Protein Expression Level in Rat’s Heart Failure Model after Myocardial Infarction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 16, Pages: 206-209(2013)
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- DOI：10.11653/syfj2013160206
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- Published：2013
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HU Yuan-hui, DU Bai, FANG Su-ping, et al. Effect of Xinfukang Oral Liquid on CK-MM mRNA and CK-MM Protein Expression Level in Rat’s Heart Failure Model after Myocardial Infarction[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 206-209.
DOI：

HU Yuan-hui, DU Bai, FANG Su-ping, et al. Effect of Xinfukang Oral Liquid on CK-MM mRNA and CK-MM Protein Expression Level in Rat’s Heart Failure Model after Myocardial Infarction[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 206-209. DOI： 10.11653/syfj2013160206.

摘要

目的: 观察心复康口服液对心肌梗死心衰大鼠心肌组织肌型肌酸激酶同工酶(CK-MM)mRNA及CK-MM蛋白表达的影响。方法: 采用结扎SD大鼠左冠状动脉前降支复制心肌梗死后心衰大鼠模型

大鼠随机分为假手术组(Sham 组)、模型组(Model 组)、Capt 0.1 g·kg-1组(Capt 组)、心复康口服液组5.4 g·kg-1(XFK组)

分别于术后 24 h 灌胃给药

给药4周末

用Real-time RT-PCR方法

Western blotting方法检测心肌细胞能量穿梭关键酶CK-MM mRNA和蛋白表达水平。结果: ①与假手术组比较

模型组左心室心肌CK-MM的mRNA表达水平下调

在第4周末

统计学有显著性意义(P<0.01);与模型组比较

Capt组、XFK组心肌CK-MM mRNA、蛋白表达均上调

在第4周末

统计学有显著性意义(P<0.01)。②与Captopril组比较

XFK组心肌CK-MM mRNA表达水平在第4周末下调

统计学有显著性意义(P<0.05);XFK组心肌CK-MM蛋白表达水平在第4周末比较无显著性意义。结论: 心复康口服液可上调心梗后心衰大鼠心肌细胞CK-MM mRNA的表达

促进CK-MM蛋白含量增加

达到改善CHF心肌细胞能量穿梭紊乱

重塑心梗后心衰大鼠心肌能量代谢作用。

Abstract

Objective: To study the effect of Xinfukang(XFK)oral liquid on MM isoenzyme of creatine kinase(CK-MM) mRNA and CK-MM protein expression level in rat of heart failure model after myocardial infarction. Method: The rat heart failure mode after myocardial infarction was created by ligating the left anterior descending coronary arteries. All the rats were randomly divided into 4 groups:sham group

model group

XFK 5.4 g·kg-1 group and captopril 0.1 g·kg-1 group. After 24 hours of modeling

rats were ig given XFK and captopril for 4 weeks

Cardiac function

myocardial ultrastructure and key enzymes of myocardial energy shuttle

CK-MM mRNA and protein expression level were separately measured in all groups. Result: ①Compared with model group

in captopril group and XFK group CK-MM mRNA and protein expression increased (P<0.01). ②Compared with captopril group

in XFK group CK-MM mRNA expression level decreased (P<0.05). In XFK group CK-MM protein expression has no significant change. Conclusion: XFK oral liquid can effectively increase the expression level of CK-MM mRNA

as well as the protein content of CK-MM to improve myocardial energy shuttle and remodel the process of myocardial energy metabolism function.

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