DU Gang, LI Lin, ZHANG Bo, et al. Effect of Platelet Rich Plasma Combined with Bone Marrow Mesenchymal Stem Cells in Treatment of Rabbit Femoral Head Necrosis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 213-216.
DOI:
DU Gang, LI Lin, ZHANG Bo, et al. Effect of Platelet Rich Plasma Combined with Bone Marrow Mesenchymal Stem Cells in Treatment of Rabbit Femoral Head Necrosis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 213-216. DOI: 10.11653/syfj2013160213.
Effect of Platelet Rich Plasma Combined with Bone Marrow Mesenchymal Stem Cells in Treatment of Rabbit Femoral Head Necrosis
Objective: To study effect of platelet rich plasma(PRP) combined with bone marrow mesenchymal stem cells(BMSCs) in treatment of rabbit femoral head necrosis. Method: Fifty rabbits were randomly divided into normal group
model group
PRP group (3%)
BMSCs group (1×106/mL) and combination group. The model of rabbit femoral head necrosis was made by operation. After 4 weeks of operation
the PRP group was implanted the PRP
the BMSCs group was implanted the BMSCs
the combination group implanted the PRP and BMSCs. Normal group was not treated. The rabbit femoral head necrosis were observed by HE trichrome coloration after 12 weeks of operation. The change of the trabeculae of bone
bone cells in the bone
fat cells were observed by light microscopy. Then PDGF protein in the rabbit femoral head was tested by Western blot. Result: Compared with the normal group
the trabecular of bone became thinner
decreased in bone cells
fat cells increased
empty osteocyte lacuna in the trabeculae were increased in model group (P<0.05). Compared with the model group
in all treatment groups trabecular bone became thicker
bone cells increased
fat cells decrease
empty osteocyte lacuna in the trabeculae were decreased and improved the expression of PDGF in the rabbit femoral head (P<0.05). Conclusion: PRP combined with BMSCs can effectively induce bone regeneration
improved the expression of PDGF and improve bone tissue recovery.
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