Effects of Vinegar Processing  on the Expression and Function of P-glycoprotein in Caco-2 Cells

LI Shu-wen; HU Zhi-fang

doi:10.11653/syfj2013160217

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Effects of Vinegar Processing on the Expression and Function of P-glycoprotein in Caco-2 Cells

更新时间：2024-01-04

- Effects of Vinegar Processing on the Expression and Function of P-glycoprotein in Caco-2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 16, Pages: 217-219(2013)
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- DOI：10.11653/syfj2013160217
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- Published：2013
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LI Shu-wen, HU Zhi-fang. Effects of Vinegar Processing on the Expression and Function of P-glycoprotein in Caco-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 217-219.
DOI：

LI Shu-wen, HU Zhi-fang. Effects of Vinegar Processing on the Expression and Function of P-glycoprotein in Caco-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 217-219. DOI： 10.11653/syfj2013160217.

摘要

目的: 通过研究香附醋制前后对Caco-2 细胞P-糖蛋白(P-gp)功能和表达的影响

初步阐明"香附醋制增效"理论的机制。方法: 采用Caco-2细胞模型

细胞以2×105/mL的密度接种于Trenswell 6孔板

加入体积分数分别为5%

10%

20%的香附生品及醋制品含药血清培养24 h

流式细胞仪检测香附醋制前后对Caco-2 细胞Pgp 功能的影响;免疫组化法检测P-糖蛋白的表达。结果: 香附生品低、中、高浓度可使细胞内地高辛累积增加8.25%

9.57%

16.84%

醋制品低、中、高浓度可使细胞内地高辛累积增加22.44%

29.12%

33.09%。二者均可使细胞内地高辛累积增加

并使P-gp 表达的阳性率降低

对P-gp 功能和表达均呈现出抑制作用

且醋制品作用更加明显。结论: 香附醋制后可增加其抑制Caco-2 细胞P-gp 功能和表达的作用

从而促进P-gp底物的吸收。

Abstract

Objective: To explore the effect of vinegar processing Cyperus rotundus on the expression and function of P-glycoprotein(P-gp)in Caco-2 cells. Method: The function of P-gp in Caco-2 cells was measured by FCM and the expression was by immunohistochemistry after the interaction between the cell(2×105/mL) and the medicine of concentration(5%

10%

20%). Result: The low

medium

high concentration of crude C. rotundus could increased 8.25%

9.57%

16.84% of the accumulation of digaoxi

vinegar processing C. rotundus could increase 22.44%

29.12%

33.09% of the accumulation of digaoxi.Serum containing crude and vinegar processing C. rotundus could remarkedly increase the accumulation of digaoxi and decrease the positive rate of P-gp's expression. Conclusion: C. rotundus after vinegar processed can promote the function of inhibiting the expression and function of P-gp in Caco-2 cells.

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