Objective: Study on the mechanism of Zhishi Xiaopi Wan treatment on Gastric cancer from cell proliferation

apoptosis and cycle. Method: Complete medium was mixed in negative control group;1×104 cells/wells were seeded at 96 wells plate and divided into negative control

Zhishi Xiaopi Wan in 0.05

0.125

0.5

2

4 g·L-1 concentration groups;the cells were treated with drugs for 24

48

72 h

and cell proliferation was detected by MTT method; 1×105 cells/wells were seeded in 6 wells plate

and the drugs of low(0.125 g·L-1)

medium(0.5 g·L-1)

high(2 g·L-1) concentration respectively were put into wells for 48

72 h

then the cell cycle and apoptosis of gastric cancer SGC-7901 cells were detected by flow cytometry(FCM). Result: Gastric cancer SGC-7901 cells were treated by the 0.125

0.5

2 g·L-1 of Zhishi Xiaopi Wan

and its inhibition rate is in dose and time dependent. Zhishi Xiaopi Wan could increase SGC-7901 cells in G0-G1 phase percentage and promote apoptosis; The high

medium concentration was stronger than the lows. Conclusion: Zhishi Xiaopi Wan can inhibit the proliferation of SGC-7901 cell and there is in dose and time dependent

which mechanism may be related to drug block of cells to G0/G1 phase and induce cell apoptosis.