Uptake Determination of Guanfu Base I in Caco-2 Cells

WANG Rui; YU Tong; CHU Yan-le; WANG Lin-xi; LI Xiao-tian

doi:10.11653/syfj2013160282

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Uptake Determination of Guanfu Base I in Caco-2 Cells

更新时间：2024-01-04

- Uptake Determination of Guanfu Base I in Caco-2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 16, Pages: 282-285(2013)
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- DOI：10.11653/syfj2013160282
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- Published：2013
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WANG Rui, YU Tong, CHU Yan-le, et al. Uptake Determination of Guanfu Base I in Caco-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 282-285.
DOI：

WANG Rui, YU Tong, CHU Yan-le, et al. Uptake Determination of Guanfu Base I in Caco-2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(16): 282-285. DOI： 10.11653/syfj2013160282.

摘要

目的: 研究关附壬素在Caco-2细胞上的摄取特性。方法: 采用液相色谱质谱联用(LC-MS)测定法

COSMOSIL C18柱(150 mm×2.0 mm

5 μm)

流动相:乙腈-水(含0.2%冰乙酸)30:70

流速0.2 mL·min-1

电喷雾离子化(ES1)方式

采用选择性离子检测法

检测离子为正离子

关附壬素的选择性离子:[M+H]

m/z 388。考察了温度、药物浓度、时间和pH对关附壬素在Caco-2细胞上摄取的影响。结果: 关附壬素在0.2~50.0 μmol·L-1内呈良好的线性关系(r=0.996 7)

最低定量限为0.2 μmol·L-1。关附壬素在Caco-2细胞上的摄取具有一定的浓度、时间和pH依赖性。结论: 该方法灵敏、操作简单

选择性强

关附壬素在Caco-2细胞上摄取良好

其摄取表现为被动扩散。

Abstract

Objective: To study the uptake status of Guanfu Base I(GFI)in Caco-2 ceils. Method: To establish Liquid Chromotography with Mass Spectrometry(LC-MS) assay

A LC procedure was carried out using an C18(150 mm×2.0 mm

5 μm)and an eluate of 0.2% acetic acid-acetonitrile (30:70)at the flow rate of 0.2 mL·min-1.The MS portion was set with ESI ionsource in the positive ion polarity mode and the selective ion monitoring(SIM).A protonated molecules[M+H] were detected at m/z 388.10 for GFI.The GFI concentrations in Caco-2 cells at various time

concentration

temperature and pH values were measured. Result: The linearity of GFI concentrationcurve was found in the range of 0.2-50.0 μmol·L-1 (r=0.996 7).The minimal detection limit was 0.2 μmol·L-1.The GFI uptakes in Caco-2 cells obeyed a passive diffusion in a time

concentration and pH-dependent manner. Conclusion: This sensitive and specific LC-MS assay can be applied to determine GFI concentrations in Caco-2 cells.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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