Optimization of Ultrasound-Assisted Extraction Technology for Betulinic Acid from Forsythiae Fructus by Response Surface Methodology

ZHANG Sheng; WANG Yu; WANG Shao-ming; WEI Huan-yong; GUO Sheng-lei; YAN Xiu-feng

doi:10.11653/syfj2013170015

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Optimization of Ultrasound-Assisted Extraction Technology for Betulinic Acid from Forsythiae Fructus by Response Surface Methodology

更新时间：2024-01-04

- Optimization of Ultrasound-Assisted Extraction Technology for Betulinic Acid from Forsythiae Fructus by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 17, Pages: 15-18(2013)
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- DOI：10.11653/syfj2013170015
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- Published：2013
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ZHANG Sheng, WANG Yu, WANG Shao-ming, et al. Optimization of Ultrasound-Assisted Extraction Technology for Betulinic Acid from Forsythiae Fructus by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 15-18.
DOI：

ZHANG Sheng, WANG Yu, WANG Shao-ming, et al. Optimization of Ultrasound-Assisted Extraction Technology for Betulinic Acid from Forsythiae Fructus by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 15-18. DOI： 10.11653/syfj2013170015.

摘要

目的: 应用响应面法优化超声辅助提取连翘中桦木酸的工艺条件。方法: 在单因素试验基础上

采用Box-Behnken试验设计和响应面分析法考察乙醇体积分数、提取温度、液固比对桦木酸提取率的影响。HPLC测定桦木酸含量

Thermo Hypersil GOLD AQ-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水(9:1)

流速1.2 mL·min-1

柱温25℃

进样量20 μL

检测波长210 nm。结果: 最佳超声提取工艺为乙醇体积分数80%

提取温度37℃

液固比32 mL·g-1

桦木酸提取率(4.28±0.05) mg·g-1

与模型预测值(4.30 mg·g-1)基本一致。结论: 该工艺合理、可靠

有效成分提取率高。

Abstract

Objective: To optimize ultrasonic-assisted extraction technology of betulinic acid from Forsythiae Fructus using response surface methodology. Method: On the basis of single factor tests

Box-Behnken experimental design and response surface methodology was adopted to optimize extraction conditions with the concentration of ethanol

extraction temperature and solid-liquid ratio as factors.HPLC was used to determine the content of betulinic acid from Forsythiae Fructus

chromatographic conditions were as follows:Thermo Hypersil GOLD AQ-C18 column (4.6 mm×250 mm

5 μm)

acetonitrile-water (9: 1) as mobile phase

flow rate of 1.2 mL·min-1

injection volume 20 μL

detection wavelength at 210 nm. Result: Optimal ultrasound-assisted extraction technology of betulinic acid from Forsythiae Fructus was as following:the volume fraction of ethanol 80%

extraction temperature 37℃

liquid-solid ratio 32 mL·g-1.The actual value of betulinic acid yield was (4.28±0.05) mg·g-1

which was consistent with the predicted value of 4.30 mg·g-1. Conclusion: This optimized extraction process was reasonable and reliable with high yield of effective components.

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