Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC

DAI Li-ping; XIE Xiao-long; LU Yan-qing; WANG Juan

doi:10.11653/syfj2013170127

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Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC

更新时间：2024-01-04

- Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 17, Pages: 127-129(2013)
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- DOI：10.11653/syfj2013170127
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- Published：2013
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DAI Li-ping, XIE Xiao-long, LU Yan-qing, et al. Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 127-129.
DOI：

DAI Li-ping, XIE Xiao-long, LU Yan-qing, et al. Quantitative Determination of Paeonol and Paeoniflorin in Extraction of Danshao Formula by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 127-129. DOI： 10.11653/syfj2013170127.

摘要

目的: 建立HPLC同时测定丹芍方提取物中丹皮酚、芍药苷含量的方法。方法: 采用ODSHYPERSIL色谱柱(4.6 mm×250 mm

5 μm)

乙腈-水梯度洗脱

柱温32℃

流速0.8 mL·min-1

检测波长230

274 nm。结果: 丹皮酚、芍药苷分别在0~0.202 1

0~27.960 0 μg线性关系良好

平均回收率分别为97.40%

101.35%

RSD分别为1.18%

0.48%。结论: 3批样品测定结果表明

建立的方法简便、准确、重复性好

可以用于丹芍方提取物中丹皮酚、芍药苷的含量测定。

Abstract

Objective: To establish a method of HPLC for quantitative determination of paeonol and paeoniflorin in the extraction of Danshao Formula. Method: The separation was performed on the chromatographic column of ODS HYPERSIL (4.6 mm×250 mm

5 μm)

and the mobile phase in the gradient elution program was acetonitrile-water. The column temperature was maintained at 32℃. The flow rate was 0.8 mL·min-1

and the detection wavelength was set at 274 nm and 230 nm respectively. Result: Paeonol and paeoniflorin showed good linear relationship in the range of 0-0.202 1

0-27.960 0 μg. The average recovery was 97.40% (RSD 1.18%)

101.35% (RSD 0.48%) respectively. Conclusion: The method is easy and accurate with higher repeatability

which can be used in the quality control of paeonol and paeoniflorin in the extraction of Danshao formula.

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Related Institution

Zhejiang Chinese Medical University

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Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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Jilin Huakang Pharmaceutical Co. Ltd.

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