Simultaneous Determination of Curdione and Germacrone in Xingnaojing Injection Using High Performance Liquid Chromatography

YANG Li-xin; LIU Wei-wei; GAN Guo-feng; ZHOU Fu-kang; HE Xi-rong; LI Chun

doi:10.11653/syfj2013170136

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Simultaneous Determination of Curdione and Germacrone in Xingnaojing Injection Using High Performance Liquid Chromatography

更新时间：2024-01-04

- Simultaneous Determination of Curdione and Germacrone in Xingnaojing Injection Using High Performance Liquid Chromatography
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 17, Pages: 136-139(2013)
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- DOI：10.11653/syfj2013170136
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- Published：2013
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YANG Li-xin, LIU Wei-wei, GAN Guo-feng, et al. Simultaneous Determination of Curdione and Germacrone in Xingnaojing Injection Using High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 136-139.
DOI：

YANG Li-xin, LIU Wei-wei, GAN Guo-feng, et al. Simultaneous Determination of Curdione and Germacrone in Xingnaojing Injection Using High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 136-139. DOI： 10.11653/syfj2013170136.

摘要

目的: 建立醒脑静注射液中莪术二酮及吉马酮的含量测定方法。方法: 采用HPLC对醒脑静注射液中莪术二酮和吉马酮进行含量测定。Waters symmetry C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水(60:40)

检测波长210 nm

柱温35℃

流速1.0 mL·min-1。结果: 莪术二酮在0.072 9~1.98 μg(r=0.999 9)

吉马酮在0.023 2~0.58 μg(r=0.999 9)呈良好的线性关系。莪术二酮和吉马酮的平均回收率分别为99.47%

100.2%

RSD分别为2.73%

2.94%。结论: 所建立的方法简便

重复性好

可用于醒脑静注射液的质量控制。

Abstract

Objective: To establish a method for simultaneous quantitative determination of curdione and germacrone in Xingnaojing injection. Method: HPLC was used to determine the content of curdione and germacrone in Xingnaojing injection. The analysis was performed on an Waters symmetry C18 column. The mobile phase was acetonitrile-water(60: 40)

the monitoring wavelength was 210 nm

the column temperature was 35℃ and the flow rate was 1.0 mL·min-1. Result: The linear ranges of curdione and germacrone were 0.072 9-1.98 μg (r=0.999 9) and 0.023 2-0.58 μg (r=0.999 9) respectively. The average recovery of curdione and germacrone were 99.47% (RSD 2.73%) and 100.2%(RSD 2.94%) respectively

which showed the method was reliable. Conclusion: The method was simple and repeatable

and can be used for the quality control of Xingnaojing injection.

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Related Institution

Zhejiang Chinese Medical University

School of Pharmacy,Shenyang Pharmaceutical University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Institute of Material Medica,Key Laboratory of Advanced Drug Delivery of Guangdong Province,Guangdong Pharmaceutical College

Foshan Fengliaoxing Pharmaceutical Co.,Ltd

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