Determination of Hydroxyacetophenone in Qingganlidan Oral Solution

LI Xiao-mei; LIANG Yong; KANG Zhi-ying; WANG De-xiu; CAI Chun-ling; MA Yin-lian; YU Ding-rong

doi:10.11653/syfj2013170160

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Determination of Hydroxyacetophenone in Qingganlidan Oral Solution

更新时间：2024-01-04

- Determination of Hydroxyacetophenone in Qingganlidan Oral Solution
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 17, Pages: 160-162(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013170160
  CLC：
- Published：2013
- 稿件说明：
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LI Xiao-mei, LIANG Yong, KANG Zhi-ying, et al. Determination of Hydroxyacetophenone in Qingganlidan Oral Solution[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 160-162.
DOI：

LI Xiao-mei, LIANG Yong, KANG Zhi-ying, et al. Determination of Hydroxyacetophenone in Qingganlidan Oral Solution[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 160-162. DOI： 10.11653/syfj2013170160.

摘要

目的: 建立对羟基苯乙酮含量测定的方法

为清肝利胆口服液的质量控制提供依据。方法: 采用高效液相色谱法测定对羟基苯乙酮的含量

Kromasil 100-5 C18 色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.1%磷酸溶液(25:75)

流速 1.0 mL·min-1

检测波长275 nm

进样量10 μL

柱温35℃。结果: 对羟基苯乙酮在0.213~5.335 μg线性关系良好(r=0.999 9)

平均加样回收率101.86%(RSD 0.38%)。3批样品中对羟基苯乙酮含量分别为0.22

0.19

0.18 mg/支。结论: 方法专属性强、简便、准确、稳定

可用于清肝利胆口服液的质量控制。

Abstract

Objective: To establish a method for determination of hydroxyacetophenone in Qingganlidan Oral Solution and provide the basis of the quality control. Method: HPLC was used to determine the content of hydroxyacetophenone. A Kromasil 100-5 C18 (4.6 mm×250 mm

5 μm) column was used. The mobile phase was methanol-0.1% phosphoric acid (25: 75) and the velocity of flow was 1.0 mL·min-1. The detection wavelength was set at 275 nm. The injection volume was 10 μL and column temperature was kept at 35℃. Result: The calibration curve of hydroxyacetophenone was in good linearity over the range of 0.213-5.335 μg(r=0.999 9). The average recovery was 99.06% with RSD of 1.54% (n=6). The content of hydroxyacetophenone in Qingganlidan Oral Solution was 0.22

0.19

0.18 mg respectively. Conclusion: The method is simple

accurate

reliable and stable. It can be used for the quality control of Qingganlidan Oral Solution.

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