LIU Dong, HE Xiu-feng, DU Shou-ying, et al. Simultaneous Determination and Pharmacokinetics of Three Bufadienolides in Dogs[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 188-192.
DOI:
LIU Dong, HE Xiu-feng, DU Shou-ying, et al. Simultaneous Determination and Pharmacokinetics of Three Bufadienolides in Dogs[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 188-192. DOI: 10.11653/syfj2013170188.
Simultaneous Determination and Pharmacokinetics of Three Bufadienolides in Dogs
Objective: To establish a HPLC method for the assay of bufalin(BL)
cinobufagin(CBG)
resibufogenin(RBG) and study the pharmacokinetics of BL
CBG
RBG in dogs plasma. Method: The sample blood from dogs were injected Chan Su extract by 0.18 mg·kg-1 at 2
5
10
15
20
30
45
60
90 min. Protein precipitation with acetonitrile and solid-phase extraction were applied to purify plasma samples and used HPLC to determine the concentration of BL
CBG
RBG in it. The pharmacokinetics parameters were accessed by Kinetica software. Result: In this SPE-HPLC method
the separation
precision and accuracy of BL
CBG
RBG were well. The calibration curves were linear. The analytical recoveries were all more than 90%
intra and inter-day precision RSD were all less than 10.0%(n=5). The main pharmacokinetics parameters were fitted by non-compartment models. Conclusion: The SPE-HPLC method can be used to determine the concentration and to investigate the pharmacokinetics of BL
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Related Author
DU Shou-ying
HE Xiu-feng
LI Na
SUN Jing
QI Yunpeng
LI Siyuan
HU Yingbo
ZHONG Linying
Related Institution
School of Life Sciences,Beijing University of Chinese Medicine
School of Pharmacy,Shanghai University of Traditional Chinese Medicine
First Teaching Hospital of Tianjin University of TCM
The Second Affiliated Hospital of Tianjin University of TCM
School of Graduate Studies,Tianjin University of Traditional Chinese Medicine(TCM)