Research on Antibacterial Active Fraction of  against Methicillin-Resistant

PENG Fu; WAN Feng; XIONG Liang; PENG Cheng; CHEN Fang

doi:10.11653/syfj2013170217

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Research on Antibacterial Active Fraction of against Methicillin-Resistant

更新时间：2024-01-04

- Research on Antibacterial Active Fraction of against Methicillin-Resistant
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 17, Pages: 217-220(2013)
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- DOI：10.11653/syfj2013170217
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- Published：2013
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PENG Fu, WAN Feng, XIONG Liang, et al. Research on Antibacterial Active Fraction of against Methicillin-Resistant [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 217-220.
DOI：

PENG Fu, WAN Feng, XIONG Liang, et al. Research on Antibacterial Active Fraction of against Methicillin-Resistant [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 217-220. DOI： 10.11653/syfj2013170217.

摘要

目的: 研究白及对"超级细菌"——耐甲氧西林金黄色葡萄球菌(MRSA)的抑菌作用及活性部位。方法: 规范提取白及乙酸乙酯部位、正丁醇部位、萃取后水部位、醇提后水煎液部位等4个部位

采用琼脂平板二倍稀释法测定以上4个部位对金黄色葡萄球菌(MSSA)及MRSA共6株病原菌的MIC;用白及乙酸乙酯部位以两种给药方式对小鼠进行预防给药

测定白及对MRSA感染SPF小鼠的保护力。结果: 乙酸乙酯部位和正丁醇部位对受试病原菌均具明显抑菌活性

4个部位中尤以乙酸乙酯部位的抑菌活性最强

其MIC为0.065~0.26 g·L-1;且白及乙酸乙酯部位对MRSA感染小鼠有很强的保护作用

其中尤以ip 0.5 g·kg-1的保护达到100%。结论: 白及对MSSA和MRSA有明显的抑菌作用

乙酸乙酯部位是其主要的活性部位。

Abstract

Objective: To study antibacterial active fraction of Bletilla striata against methicillin-resistant Staphylococccus aureus(MRSA). Method: Minimum inhibitory concentrations (MICs) of ethyl acetate extract

n-butanol extract

water extract after extraction and water decoction after extracting alcohol from B. striata against MRSA and S. aureus(MSSA) was assayed. Oral administration and intraperitoneal injection of ethyl acetate extract from B. striata were given to mice before they are infected by MRSA. Result: Ethyl acetate extract and n-butanol extract showed obvious antibacterial activity. Ethyl acetate extract had the highest activity with MIC 0.065-0.26 g·L-1. Ethyl acetate extract could completely protect mice from MRSA through intraperitoneal injection with the concentration of 0.5 g·kg-1. Conclusion: Ethyl acetate extract is the main active fraction of B. striata against MRSA and MSSA.

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