Effect of Xiaoyao San on Prefrontal Cortex PP-2A and GSK-3 in Alzheimer's Disease Rat Model

LI Gao-shen; LIU Jian-tao; ZHAO Wei-xian; GUO Mei-zhen; LI Yi-pei; WANG Bao-wei

doi:10.11653/syfj2013170244

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Effect of Xiaoyao San on Prefrontal Cortex PP-2A and GSK-3 in Alzheimer's Disease Rat Model

更新时间：2024-01-04

- Effect of Xiaoyao San on Prefrontal Cortex PP-2A and GSK-3 in Alzheimer's Disease Rat Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 17, Pages: 244-247(2013)
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- DOI：10.11653/syfj2013170244
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- Published：2013
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LI Gao-shen, LIU Jian-tao, ZHAO Wei-xian, et al. Effect of Xiaoyao San on Prefrontal Cortex PP-2A and GSK-3 in Alzheimer's Disease Rat Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 244-247.
DOI：

LI Gao-shen, LIU Jian-tao, ZHAO Wei-xian, et al. Effect of Xiaoyao San on Prefrontal Cortex PP-2A and GSK-3 in Alzheimer's Disease Rat Model[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(17): 244-247. DOI： 10.11653/syfj2013170244.

摘要

目的: 探讨逍遥散对阿尔茨海默病大鼠模型前额叶皮质相关指标的影响。方法: 将清洁级4~5个月龄SD雄性大鼠随机分为4个组

模型组(B 组)、西药组(C 组)、中药组(D 组)分别采用D-半乳糖腹腔注射和 A-β1~42peptide双侧海马注射联合造模[用药剂量:D-半乳糖10 mL(100 mg·kg-1)

每日1次

连续注射42 d;A-β每侧海马组织1次性注射2 μL(10 μg)]

生理组(A 组)仅用等体积生理盐水做相同的造模处理。造模完成后

C、D组每日分别用奥拉西坦溶液0.75 g·kg-1和逍遥散煎剂10·g-1 ig干预

1次/d

连续28 d

用药体积均为5 mL·kg-1;A

B组采用等体积的生理盐水ig。ig结束后

4个组分别进行Y迷宫行为学检测

并对比分析。之后断头剥离前额叶皮质送检。结果: B

D两组与B组比较

其尝试次数和行为正确率均占明显优势

差异具有统计学意义(尝试次数P<0.05;行为正确率P<0.01);B组与A组比较

尝试次数和行为正确率降低

差异具有统计学意义(尝试次数P<0.05;行为正确率P<0.01);降低C、D组与A组比较差异无统计学意义。免疫组化染色显示

与B组比较

C组和D组PP-2A阳性细胞数量表达均明显增加

而GSK-3β阳性细胞数量表达均明显减少。结论: 逍遥散能改善AD大鼠的空间记忆能力

与其能够上调AD大鼠前额叶的PP-2A的表达、抑制前额叶的GSK-3β的表达有关。

Abstract

Objective: To investigate effect of Xiaoyao San on related indexes of the prefrontal cortex in rats model of Alzheimer's disease. Method: The male SD rats were randomly divided into 4 groups

model group (group B)

western medicine group (C group)

Chinese medicine group (D group) respectively by intraperitoneal injection of D-galactose and A-β1-42 peptide bilateral hippocampal injection molding (D-galactose 10 mL (100 mg·kg-1)

once a day

continuous injection for 42 d;A-β 2 μL (10 μg) once

normal group (group A) was given normal saline. After modeling

C and D groups were given oxiracetam solution and Xiaoyao San ig once for 28 days. Result: In C and D groups

compared with B group

the number of attempts and the correct behavior rate is obvious

the difference was statistically significant (P<0.05 and P<0.01);In B group

compared with A group

number of attempts and the correct behavior rate is poor

the difference was statistically significant (P<0.05 and P<0.01);there was no significant difference in C

and D groups compared with A groups. Immunohistochemical staining resultd showed that

compared with B group

C group and D group increased the expression of PP-2A positive cells significantly

while the number of GSK-3 positive cells were significantly reduced. Conclusion: Xiaoyao San can improve the spatial memory ability of AD rats

the expression is related to its up-regulatiing expression of PP-2A and inhibitory prefrontal GSK-3β.

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