Objective: To explore the effect of traditional Tibetan medicine tonic prescription Basangmu Suyou wan on erythropoiesis and elucidate the underlying mechanism in mice with joint radiation and chemical injury. Method: Using a blood cell counter

a colony forming assay and fluorescent real-time quantitative PCR

the changes in peripheral erythrocyte count

hemoglobin content

erythroid hematopoietic progenitors colony forming proportion of bone marrow cell

kidney erythropoietin (Epo)

spleen Epo receptor (EpoR) mRNA were investigated after 0.75

1.5

3.0 g·kg-1Basangmu Suyou wan was gastrically given to the mice with joint radiation and chemical injury

and compared with those in the blank group as well as the control group given physical saline. Result: The erythrocyte count of blank

modle

low dose

middle dose

high dose group were(8.92±0.30)

(8.67±0.38)

(8.85±0.49)

(9.35±0.77)

(9.61±0.30)×1012/L respectively at 14 days. The counts of 14 days middle dose and high dose group were highen than those of the blank

the modle and of low dose group (P<0.05). The hemoglobin contents were (157.00±6.78)

(145.75±37.81)

(162.09±8.57)

(166.17±9.40)

(162.00±6.07)g·L-1 respectively at 20 days aften tneetment

the content of middle dose group was higher than those of the blank and modle group (P<0.05). The bone marrow cell colong forming unit erythrocyte (CFU-E) and burst-forming unit erythroid(BFU-E) proportion in the blank

modle and the middle dose group were (56.56±6.64)

(55.18±7.07)

(64.31±9.15)/5×104 bone marrow cell(BMC)and (31.68±6.13)

(33.44±6.5)

(40.69±8.06)/5×104BMC respectively at 14 days

the proportion in the middle dose groups were more higher than those of the blank and modle groups (P<0.01). The kidney Epo mRNA expression level of the blank

the modle and the middle dose group were (1.13±0.53)

(0.92±0.20)

(2.48±1.56)2-ΔΔCtrespectively

the level in middle dose group was higher than those of the blank and modle groups (P<0.05). Conclusion: Basangmu Suyou wan promotes the kidney erythropoietin secretion

the erythroid hematopoietic progenitor proliferation and differentiation

and then induce the recovery of peripheral erythrocyte count and hemoglobin content in mice with joint radiation and chemical injury.