Optimization of Extraction Technology for Polysaccharides from Citri Reticulatae Pericarpium by Central Composite Design-Response Surface Methodology

LIU Rong; WEI Zheng; YIN Ling; FAN Dan-qing; YANG Li; CHEN Hong-ping; LIU You-ping

doi:10.11653/syfj2013180023

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Optimization of Extraction Technology for Polysaccharides from Citri Reticulatae Pericarpium by Central Composite Design-Response Surface Methodology

更新时间：2024-01-04

- Optimization of Extraction Technology for Polysaccharides from Citri Reticulatae Pericarpium by Central Composite Design-Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 18, Pages: 23-26(2013)
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- DOI：10.11653/syfj2013180023
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- Published：2013
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LIU Rong, WEI Zheng, YIN Ling, et al. Optimization of Extraction Technology for Polysaccharides from Citri Reticulatae Pericarpium by Central Composite Design-Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 23-26.
DOI：

LIU Rong, WEI Zheng, YIN Ling, et al. Optimization of Extraction Technology for Polysaccharides from Citri Reticulatae Pericarpium by Central Composite Design-Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 23-26. DOI： 10.11653/syfj2013180023.

摘要

目的: 采用星点设计-效应面法优化陈皮多糖的提取工艺。方法: 在单因素试验基础上

选取料液比、提取温度、提取时间为自变量

陈皮多糖提取率为因变量

利用星点设计模型对3个自变量各水平二项式进行拟合

确定陈皮多糖的最佳提取工艺。结果: 优选的提取工艺为料液比1:49

提取温度92 ℃

回流提取时间150 min

陈皮多糖提取率达14.77%

与预测值的偏差0.61%。结论: 星点设计-效应面法优化的陈皮多糖提取工艺简便可靠

为陈皮多糖的开发利用奠定基础。

Abstract

Objective: To optimize extraction process of polysaccharides from Citri Reticulatae Pericarpium by central composite design-response surface methodology. Method: Based on single-factor tests

with solid-liquid ratio

extraction temperature

extraction time as independent variables and yield of polysaccharides from Citri Reticulatae Pericarpium as dependent variable

multiple linear regression and binomial fitting was adopted by central composite design model

and determine optimum extraction technology. Result: The best extraction process was as following:ratio of solid to liquid 1: 49

extraction temperature 92 ℃

reflux extraction time 150 min.Yield of polysaccharides from Citri Reticulatae Pericarpium was up to 14.77%

deviation of the predictive value and the measured value was 0.61%. Conclusion: This optimized extraction process was simple and reliable

it could laid a certain foundation for development and utilization of polysaccharides from Citri Reticulatae Pericarpium.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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