PAN Qiao-dan, HUANG Yuan-he, DONG Mei-ling, et al. Optimization of Ultrasonic-Microwave Synergistic Extraction Technology of and Investigation of Its Antioxidant Activity[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 30-32.
DOI:
PAN Qiao-dan, HUANG Yuan-he, DONG Mei-ling, et al. Optimization of Ultrasonic-Microwave Synergistic Extraction Technology of and Investigation of Its Antioxidant Activity[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 30-32. DOI: 10.11653/syfj2013180030.
Optimization of Ultrasonic-Microwave Synergistic Extraction Technology of and Investigation of Its Antioxidant Activity
Objective: To optimize ultrasonic-microwave synergistic extraction technology of total triterpenes from Trichosanthes cucumeroides and investigate its antioxidant activity. Method: With the content of total triterpenes as index
single-factor tests and orthogonal test were adopted to investigate effects of extraction time
ethanol concentration
solid-liquid ratio and microwave power on extraction technology.Antioxidant activity of its extract was extimated by DPPH assay
salicylicl acid assay
ABTS assay and FRAP assay. Result: The best extraction conditions for total triterpenes from T. cucumeroides were as follows:extracted 6 min with 50 times the amount of 90% ethanol
microwave power 500 W.Scavenging DPPH·
scavenging ABTS+·and reducing Fe3+ of extract from T. cucumeroides were higher than tea polyphenol and lower than Vc
but scavenging OH·was lower than theirs. Conclusion: This optimized technology was simple and rapid
extract of T. cucumeroides showed certain antioxidant activity.
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