Simultaneous Quantitative Analysis of Three Components in  by Multi-Components Assay by Single Marker

CHEN Qing-feng; LIU Jia; QIAO Li; LIU Li-na; LONG Qing-de; WANG Ai-ming; LI Yong-jun

doi:10.11653/syfj2013180126

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Simultaneous Quantitative Analysis of Three Components in by Multi-Components Assay by Single Marker

更新时间：2024-01-04

- Simultaneous Quantitative Analysis of Three Components in by Multi-Components Assay by Single Marker
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 18, Pages: 126-130(2013)
- 作者机构：
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- DOI：10.11653/syfj2013180126
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- Published：2013
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CHEN Qing-feng, LIU Jia, QIAO Li, et al. Simultaneous Quantitative Analysis of Three Components in by Multi-Components Assay by Single Marker[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 126-130.
DOI：

CHEN Qing-feng, LIU Jia, QIAO Li, et al. Simultaneous Quantitative Analysis of Three Components in by Multi-Components Assay by Single Marker[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 126-130. DOI： 10.11653/syfj2013180126.

摘要

目的: 建立透骨香中儿茶素、白珠树苷、滇白珠苷A的一测多评含量测定方法。方法: 采用高效液相色谱

以中药透骨香为研究对象

以儿茶素为内参物

建立其与白珠树苷、滇白珠苷A的相对校正因子

并进行含量测定

实现一测多评。同时采用外标法测定28批透骨香中儿茶素、白珠树苷、滇白珠苷A的含量

验证一测多评法的准确性。结果: 建立的校正因子重复性良好

采用校正因子计算的含量值与外标法实测值之间无显著差异。结论: 以外标法测定儿茶素

利用相对校正因子实现对白珠树苷和滇白珠苷A测定是准确的、可行的

一测多评法可以用于特定的不同类型成分间的测定。

Abstract

Objective: To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining catechin

gaultherin and gaultheroside A in G. yunnanensis. Method: A HPLC method was developed as QAMS to determine the three principal components in Gaultheria yunnanensis. Catechin was selected as the internal reference substance

and 2 relative correction factors (RCFs) to catechin were calculated. The method was evaluated by comparison of the quantitative results between external standard method and QAMS method. Result: No siginificant differences between the quantitative results of QAMS method and external standard method were observed. Conclusion: It is feasible and suitable to determine catechin

gaultherin and gaultheroside A in Gaultheria yunnanensis by QAMS

and this method can be used for a certain different types of compounds.

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