Determination of Aconitine, Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC

REN Xin-xin; LI Xing-hua; YE Sha; MA Xiao-ya; LEI Jian-lin; LI Wan-jing; DUAN Zong-ming

doi:10.11653/syfj2013180131

您当前的位置：

首页 >

文章列表页 >

Determination of Aconitine, Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC

更新时间：2024-01-04

- Determination of Aconitine, Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 18, Pages: 131-134(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013180131
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
REN Xin-xin, LI Xing-hua, YE Sha, et al. Determination of Aconitine, Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 131-134.
DOI：

REN Xin-xin, LI Xing-hua, YE Sha, et al. Determination of Aconitine, Mesaconitine and Hypaconitine in Fumailing Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 131-134. DOI： 10.11653/syfj2013180131.

摘要

目的: 建立复脉灵胶囊中乌头碱、新乌头碱、次乌头碱的含量测定方法。方法: 采用HPLC

流动相甲醇-0.5%的三乙胺(70:30)

Aglient TC-C18色谱柱(4.6 mm×250 mm

5 μm)

检测波长235 nm

流速 1.0 mL·min-1

柱温室温。结果: 乌头碱的进样量在0.080 8~0.808 0 μg具有良好的线性关系(r=0.999 6)

平均回收率98.40%

RSD 2.84%;新乌头碱的进样量在0.866~4.330 μg具有良好的线性关系(r=0.999 7)

平均回收率98.04%

RSD 2.05%;次乌头碱的进样量在0.089~0.890 μg具有良好的线性关系(r=0.999 8)

平均回收率98.71%

RSD 2.34%。结论: 方法操作简便、灵敏度高、结果准确

可作为复脉灵胶囊的含量测定方法。

Abstract

Objective: To establish a method for the determination of aconitine

mesaconitine and hypaconitine in Fumailing Capsules. Method: The aconitine

mesaconitine and hypaconitine in the capsules was determined by HPLC on the Aglient TC-C18column (4.6 mm×250 mm

5 μm) at room temperature. The mobile phase was consisted of methanol-0.05% triethylamine (70: 30). The flow rate was 1.0 mL·min-1 minand the detection wavelength was set at 235 nm. Result: The content of aconitine had a good linearity in the range of 0.080 8-0.808 0 μg (r=0.999 6)

the average recovery was 98.40%

RSD was 2.84%. The content of mesaconitine had a good linearity in the range of 0.866-4.330 μg (r=0.999 7)

the average recovery was 98.04% and RSD was 2.05%. The content of hypaconitine had a good linearity in the range of 0.089-0.890 μg (r=0.999 8)

the average recovery was 98.71%

and RSD was 2.34%; Conclusion: This method was simple

reproducible and accurate

and it can be used for determination of Fumailing Capsules.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Enrichment and Purification of Alkaloids from with D101 Macroporous Resin

Study on Dynamic Differences of Alkaloid in Various Tissues of

Determination of Mesaconitine,Hypaconitine, Aconitine in Bitong Medicated Wine

Simultaneous Determination of Aconitine, Hypaconitine and Mesaconitine in Sini Decoction by RP-HPLC

Pharmacokinetics of Three Alkaloids from Shiwuwei Rupeng Pills in Rats by UPLC-MS/MS

Related Author

PENG Tuo-hua

ZHANG Shao-jun

ZHONG Shi-shun

YANG Yi-da

YANG Tong

OU Shui-ping

ZHENG Qin

WANG Sen

Related Institution

Institute of Chinese Materia Medica,China Academy of TCM

中国中医研究院中药研究所北京100700

Department of Pharmacy,Jiangxi University of Traditional Chinese Medicine

Beijing Zhongyan Tongrentang Pharmaceutical Research and Development Co. Ltd.

Beijing Tongrentang Science and Technology Development Co. Ltd.

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰