Determination of Saccharide in Extracts of  by One-step Phenol-Sulfuric Acid Method

YANG Zhe-xuan; ZHOU Li-hong; ZHANG Shun-nan; YE Zheng-liang; LIU Wen-yuan

doi:10.11653/syfj2013180139

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Determination of Saccharide in Extracts of by One-step Phenol-Sulfuric Acid Method

更新时间：2024-01-04

- Determination of Saccharide in Extracts of by One-step Phenol-Sulfuric Acid Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 18, Pages: 139-142(2013)
- 作者机构：
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- DOI：10.11653/syfj2013180139
  CLC：
- Published：2013
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YANG Zhe-xuan, ZHOU Li-hong, ZHANG Shun-nan, et al. Determination of Saccharide in Extracts of by One-step Phenol-Sulfuric Acid Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 139-142.
DOI：

YANG Zhe-xuan, ZHOU Li-hong, ZHANG Shun-nan, et al. Determination of Saccharide in Extracts of by One-step Phenol-Sulfuric Acid Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 139-142. DOI： 10.11653/syfj2013180139.

摘要

目的: 建立白芍提取物中中总糖含量的测定方法。方法: 通过单因素试验确定实验的最大吸收波长

硫酸浓度

显色液用量

加热时间和显色时间。实验条件确定后

从加样回收率

重复性

线性和范围几方面进行方法学验证。同时

将优化后的方法与传统方法进行对比。结果: 最佳实验条件为:硫酸与水的体积比为5:1;显色液体积为5 mL;加热时间为50 min;显色时间为15 min。在最佳实验条件下

葡萄糖标准溶液在0.019~0.189 mg线性关系良好

回归方程为Y =7.488 X-0.000 2(R2=0.999 5)。三个比例的加样回收率为102.00%

102.15%

95.86%

RSD分别为2.08%

1.51%

2.24%;6份样品的重复性RSD为2.4%。结论: 该方法准确可靠

操作简单

也可用于其他中药提取物的质量控制。

Abstract

Objective: The aim is to establish the determination method of saccharide in Pareonia albiflota extracts. Method: The volume ratio of sulfuric acid and water

the volume of color liquid

100 ℃ water bath time

and ice bath time were studied by single-factor experiment. Result: The optimum experimental conditions were as following: 5: 1 of the volume ratio of sulfuric acid and water

5 mL color liquid

100 ℃ water bath for 50 min

and ice bath for 15 min. In the optimum experimental conditions

the standard solutions of glucose possessed good linear with absorbance when its concentration was 0.019-0.189 mg. The linear equation was Y=7.488X-0.000 2(R2=0.999 5). The recovery ratio of three degrees was 102%

102.15%

95.86%

the RSD was 2.08%

1.51%

2.24%. The RSD of six samples was 2.4%. Conclusion: The method is simple

accurate and reliable. It can be used for the quality control of traditional Chinese medicine.

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