Objective: From the cell proliferation

cycle and apoptosis researching the mechanism which Xuanfu Guilian prescription treats esophageal cancer. Method: Esophageal carcinoma cells (Eac9706) in vitro were cultured

and the cell proliferation inhibitory rate of Xuanfu Guilian prescription were detected with MTT assay to observe the time-and dose-effect; 1×105 cells/wells were seeded in 6 wells plate and respectively mixed in Xuanfu Guilian prescription extracts (15

25

60 mg·L-1);after cultured 48 h

the cell cycle and apoptosis of Eac9706 were detected by flow cytometry(FCM). Result: Xuanfu Guilian prescription can inhibit strongly the proliferation of Eca9706 cell

and have dose dependent

50% inhibition concentration(IC50) 58.11 mg·L-1. The inhibition rate of concentration 15

25

60 mg·L-1 increased on time in 96 h. Early and late apoptosis rate of treatment group were significantly increaser than the negative(P<0.05). The cells rate of G0/G1 also increased significantly(P<0.05)

and the 60 mg·L-1 group appeared apoptosis peak. Conclusion: Xuanfu Guilian prescription can inhibit the proliferation of Eac9706 cells and there is in dose and time dependent

which mechanism may be related to drug block of cells to G0/G1 phase and induce cell apoptosis.