CUI Bing-bing, CHENG Li-fang, HOU Li-jing, et al. Effect of Ursolic Acid on Inhibiting Growth and Apoptosis in Human Hepatoma SMMC-7721[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 181-184.
DOI:
CUI Bing-bing, CHENG Li-fang, HOU Li-jing, et al. Effect of Ursolic Acid on Inhibiting Growth and Apoptosis in Human Hepatoma SMMC-7721[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 181-184. DOI: 10.11653/syfj2013180181.
Effect of Ursolic Acid on Inhibiting Growth and Apoptosis in Human Hepatoma SMMC-7721
Objective: To discuss the inhibition mechanism of ursolic acid on inhibiting the growth of human carcinoma cell SMMC-7721. Method: Human hepatocellular carcinoma cell SMMC-7721 were co-cultured with different doses of ursolic acid for 24 h and 72 h
respectively. The absorbance value was evaluated by neutral red staining method
evaluation of ursolic acid on the proliferation of hepatoma cells; and the apoptosis was detected by flow cytometry. Result: Different concentrations of ursolic acid on the proliferation of SMMC-7721 cells showed obvious inhibition effect (P<0.05 or P<0.01) with a time and dose dependent manner. The half inhibitory concentration (IC50) of ursolic acid on SMMC-7721 cell strain was 12.59 mg·L-1 and 8.32 mg·L-1
respectively. The result indicated that the apoptosis rate was up to 14.07%
ursolic acid arrested SMMC-7721 the cell strain to S by flow cytometry with a dose-dependent manner. Conclusion: Ursolic acid on the proliferation of SMMC-7721 hepatoma cell line has the obvious inhibitory action
antitumor mechanism may be related to induction of cell apoptosis and cell cycle arrest in S phase.
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