Protective Effects of Flavonoids Extracted from Cuscutae Semen on Human Umbilical Vein Endothelial Cells Injured by HO

LIU Hai-yun; CUI Yan-ru; WU Qing-hua; WANG Xiao-min

doi:10.11653/syfj2013180215

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Protective Effects of Flavonoids Extracted from Cuscutae Semen on Human Umbilical Vein Endothelial Cells Injured by HO

更新时间：2024-01-04

- Protective Effects of Flavonoids Extracted from Cuscutae Semen on Human Umbilical Vein Endothelial Cells Injured by HO
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 18, Pages: 215-218(2013)
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- DOI：10.11653/syfj2013180215
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- Published：2013
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LIU Hai-yun, CUI Yan-ru, WU Qing-hua, et al. Protective Effects of Flavonoids Extracted from Cuscutae Semen on Human Umbilical Vein Endothelial Cells Injured by HO[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 215-218.
DOI：

LIU Hai-yun, CUI Yan-ru, WU Qing-hua, et al. Protective Effects of Flavonoids Extracted from Cuscutae Semen on Human Umbilical Vein Endothelial Cells Injured by HO[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(18): 215-218. DOI： 10.11653/syfj2013180215.

摘要

目的: 探讨菟丝子总黄酮对H2O2损伤的人脐静脉内皮细胞(HUVECs)的保护作用。方法: 于37 ℃、5%CO2、95%空气饱和湿度的培养箱内体外培养内皮细胞

将细胞分为4组

即正常对照组:加入DMEM培养液(pH 7.2~7.4)、菟丝子对照组 (菟丝子0.5 mg·L-1)、模型组(过氧化氢终浓度为200 mmol·L-1)、菟丝子+过氧化氢组(菟丝子0.5 mg·L-1

过氧化氢200 mmol·L-1)

研究菟丝子总黄酮对受损血管内皮细胞内超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、乳酸脱氢酶(LDH)释放情况、谷胱甘肽过氧化物酶(GSH-Px)活性及对细胞形态学的影响。结果: 菟丝子总黄酮(0.5 mg·L-1)能明显减轻H2O2对HUVECs所致的氧化损伤

可明显改善氧化损伤的HUVECs形态学变化

H2O2模型组(200 mmol·L-1)与空白组比较

LDH释放和MDA含量显著升高(P<0.01

P<0.05)

SOD释放水平和GSH-Px活力显著下降(P<0.01)

菟丝子(0.5 mg·L-1)+H2O2(200 mmol·L)组与H2O2模型组(200 mmol·L-1)比较

LDH释放和MDA含量显著下降(P<0.01

P<0.05)

SOD释放水平和GSH-Px活力显著升高(P<0.01)。结论: 菟丝子总黄酮对H2O2诱导的HUVECs的损伤具有保护作用。

Abstract

Objective: To study the protective effects of flavonoids extracted from Cuscutae Semen (FCS) on human umbilical vein endothelial cells injured by H2O2. Method: Vein endothelial cells were cultured in vitro and divided into four groups which was normal control

FCS control

model

FSC+H2O2respectively. We tested the influence of FCS on the endothelial cells oxidative damage induced by H2O2 the indexes of superoxide dismutase(SOD)

malanyldiadehyde(MDA)

lactate dehydrogenase(LDH)

glutathione peroxidase(GSH-Px)and apoptosis were measured. Result: FCS could remarkable lessen oxidative damage of endothelial cell induced by H2O2

compared with normal control groups

the levels of LDH and MDA in model group remarkably increased and the activity of SOD and GSH-Px obviously decreased;while the groups pretreated by FCS

compared with model group

the levels of LDH and MDA obviously decreased and the activity of SOD and GSH-Px increased. Conclusion: FCS has protective effects on human umbilical vein endothelial cells injured by H2O2.

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