Objective: To investigate the anti-asthmatic mechanism of compound Chuanbei granules (CCB)in ovalbumin-mediated allergic asthma model of guinea pig. Method: Seventy guinea pigs were randomly divided into normal control

model

erchen pill(2.325 g·kg-1)

dexamethasone 9.30×10-4g·kg-1 and CCB granule 0.67

1.34

2.68 g·kg-1 groups(n=10). Each group of male and female ratio was 1: 1. On the first day of the test

the guinea pigs were sensitized by intra-peritoneal injection of 10% ovalbumin (OVA) saline solution. 13 days after sensitization

corresponding drugs were given for 7 days

the model group and normal control group given distilled water

15 days later excitation with 1% ovalbumin was carried out.After treating with the CCB granules

latent period of asthma was recorded

the amount of eosinophils(EOS) was detected

the content of thromboxane B2 (TXB2) and leukotriene B4(LTB4) were examined. Result: Compared with normal control group

in model group guinea pig serum TXB2

LTB4 level was significantly increased(P<0.01)

the increased eosinophils were seen (P<0.01). Compared with model group

the CCB granules 1.34

2.68 g·kg-1 could reduce serum TXB2

LTB4 content (P<0.05

P<0.01). The CCB granule 0.67

1.34

2.68 g·kg-1 dose reduced the total number of eosinophils around the small airways of asthma guinea pig (P<0.01); and prolong the incubation period of asthma guinea pig (P<0.05

P<0.01). Conclusion: The CCB granules could play anti-asthmatic effects. The results suggest that its functional mechanism may be related to the serum inflammatoty mediators.