Objective: To evaluate the anti-metastasis effect and the inhibition of the tube-like structure formation of human umbilical vein endothelial cells of isoliquiritigenin and glabridin. Method: The sulforhodamine B (SRB) assay was used

and the mouse melanoma B16F1 and vascular endothelial ECV304 cells in logarithmic phase of growth were randomly divided into drug groups and control group

treated with the concentration of 20

40

60

80

100

120 μmol·L-1 isoliquiritigenin or glabridin

intervention durations were 48 h accordingly. And the scratch wound assay

gelatin zymography

enzyme-linked immunosorbent (ELISA) assay

acridine orange/othidium bromide (AO/EB) double staining and tube-like structure formation test were used to observe and compare the antitumor and anti-metastasis ability. Result: Inhibition rate of the B16F1 and ECV304 were enhanced along with increase of isoliquiritigenin and glabridin concentration; glabridin against melanoma cell migration

matrix metal proteinase-2 (MMP-2) expression and the ability of inhibiting tube-like structure formation were slightly lower than those of isoliquiritigenin. Conclusion: Isoliquiritigenin and glabridin have strong anti-metastasis activities

but anti-metastasis effect of glabridin was weaker than that of isoliquiritigenin within 80 μmol·L-1.