Objective: To study 3-hydroxybenzoic acid in Artemisiae Argyi Folium (carbonized and processed with vinegar) that come from different arrears

which provide the theoretical basis of the quality control and rational use of Artemisiae Argyi Folium (carbonized and processed with vinegar). Method: HPLC method

the analytical column was Prevail C18 column (4.6 mm×250 mm

5 μm). The mobile phase was acetonitrile-0.1% phosphate (15: 85). The flow rate was 1.0 mL·min-1. The detection wavelength was 240 nm and the column temperature was set at 25 ℃. Result: There was good linearity between the peak areas and concentration at the ranges of 0.010 02-0.501 00 μg for 3-hydroxybenzoic acid (r=1.000 0). The average recovery (n=9) was 97.39% with RSD 0.99%. Conclusion: The method is accurate

simple and repeatable. The 3-hydroxybenzoic acid in Artemisiae Argyi Folium (carbonized and processed with vinegar) of different arrears has significant difference.