Objective: To compare quality differences of compound Danshen decoction(CDD) prepared by three different methods

such as traditional marmite

high-pressure decocting machine and double decocting non-pressurized machine. Method: CDD was prepared by traditional marmite

high-pressure decocting machine and double decocting non-pressurized machine

respectively.The content of salvianolic acid B in decoction was determined by HPLC

chromatographic conditions were as follows:LunaC18 column(4.6 mm×250 mm

5 μL)

mobile phase of acetonitrile-methanol-formic acid-water(10:30:1:59)

detection wavelength of 286 nm

flow rate 1.0 mL·min-1

injection volume 10 μL. Result: The contents of salvianolic acid B in compound Danshen decoction prepared by traditional marmite

high-pressure decocting machine and double decocting non-pressurized machine were 2.534

2.673

2.766 g·L-1 with RSD of 3.32%

1.15%

1.25%(n=5)

respectively. Conclusion: Double decocting non-pressurized machine was more suitable for preparation of compound Danshen decoction than the other two methods.