Objective: To optimize extraction and purification processes of sedative and anti-fatigue ingredients from Acanthopanacis Senticosi Radix Et Rhizoma Seu Caulis. Method: The contents of sedative and anti-fatigue ingredients were determined by HPLC

chromatographic conditions were as follows:TechMate C18 column(4.6 mm×250 mm

5 μm)

detection wavelengths of syringin

acanthopanax E

isofraxidin at 265

209

344 nm

flow rate 1.0 mL·min-1

column temperature 30℃

injection volume 10 μL

mobile phase of acetonitrile-0.1% formic acid gradient elution.With the contents of syringin

eleutheroside E

isofraxidin as comprehensive evaluation index

orthogonal test was adopted to optimize extraction technology with ethanol concentration

extraction times and ethanol amount as factors

types of macroporous resin was screened by static adsorption-elution test

purification technology of sedative and anti-fatigue ingredients from Acanthopanacis Senticosi Radix Et Rhizoma Seu Caulis was optimized by single factor tests. Result: Optimum extraction process was as following:extracted 3 times with 10-fold the amount of 70% ethanol

1 h each time.D101 macroporous resin was selected

optimum purification technology was:sample volume 1:1.3-1:1.4

the concentration of sample solution was 0.33 g·mL-1

eluted with 3.5 BV 30% ethanol

purify of sedative and anti-fatigue ingredients 18.35%. Conclusion: Optimized extraction technology was reliable and repeatable

yield of active ingredients of Acanthopanacis Senticosi Radix Et Rhizoma Seu Caulis had satisfactory results.