Objective: To optimize submerged fermentation technology of Massa Medicata Fermentata. Method: With activity of amylase and protease as indexes

single factor test was adopted to investigate effects of flour-wheat bran ratio and different inorganic salts on fermentation technology;With activity of protease as the index

orthogonal test was used to optimize submerged fermentation technology of Massa Medicata Fermentata with fermentation temperature

shaking speed

ratio of flour-wheat bran and the amount of inorganic salts as factors. Result: Aspergillus sydowii and K2HPO4 were selected

optimum submerged fermentation technology was as following:added 12 g flour-wheat bran(1:6) and 0.15 g K2HPO4 into 150 mL water

fermentation temperature at 28℃

shaking speed 180 r·min-1. Conclusion: This optimized fermentation technology was stable and feasible

it could provide a reference for fermentation processing technology of Chinese materia medica.