Objective: To compare differences of efficacy against hepatic injury and contents of major ingredients among Americana Linn extracts prepared by different processes. Method: With water and ethanol as solvents

Americana Linn was extracted by reflux method

smashing method and magnetic stirring method

respectively.Efficacy was tested with acute hepar-injured mice model caused by CCl4

the contents of total peptides and free-amino acid were determined by Bradford and Ninhydrin colorimetric method

combining with cream rate

differences among processes was investigated by calculating weighted score. Result: Water smashing-extract could obviously resist increasing of MDA

ALT and AST in serum and relieve pathological changes of acute hepar-injured mice caused by CCl4.In each extracts

cream rates were 16.64%

20.84%

16.19%

15.75%

19.53% and 16.82%

respectively;The contents of total peptides were 0.82%

0.03%

0.96%

0.02%

0.64%

0.02%

respectively;The contents of free amino acid were 1.99%

1.11%

2.12%

1.15%

2.10%

1.03%

respectively;Composite scores were 90.64

37.31

99.46

42.53

82.42

39.00

respectively. Conclusion: Water smashing-extract of Americana Linn had a good efficacy against hepatic injury and a high score of technic indexes.