Objective: To establish a method for simultaneous determination of salviamolic acid B

tanshinoneⅡA and ferulic acid from Lemai dispersible tablets

then investigate in vitro dissolution characteristics of these three ingredients. Method: HPLC was adopted to determine the contents of salviamolic acid B

tanshinoneⅡA and ferulic acid

chromatographic conditions were as follows:AlltimaTM ODS C18 column(4.6 mm×150 mm

5 μm)

mobile phase of acetonitrile-0.5% acetic acid gradient elution

flow rate 1.0 mL·min-1

detection wavelength of 320(0-10 min)

286(10-20 min)

270(20-30 min) nm

column temperature at 30℃

injection volume 20 μL.In vitro dissolution was determined by small cup method

with 0.1 mol·L-1HCl as dissolution medium

rotate speed was 50 r·min-1and dissolving time was 60 min

accumulative dissolution of salviamolic acid B

tanshinoneⅡA and ferulic acid within different times were calculated

resemblance of release curves were compared with similarity factors(f2) method. Result: Linear range of salviamolic acid B

tanshinoneⅡA and ferulic acid were 30.15-301.48

0.15-1.52 and 0.66-6.55 mg·L-1

respectively.Compared with salviamolic acid B

f2 of tanshinoneⅡA and ferulic acid were 70.35 and 82.49

respectively. Conclusion: Dissolution of salviamolic acid B

tanshinoneⅡA had similar characteristics with ferulic acid in Lemai dispersible tablets.