The Comparison with Kjeldah and Coomassie Brilliant Blue Method on Testing Protein Content of the Polysaccharide from

LIU Yu-ming; QIAN Tian-tian; JIANG Ding-wen; HE Ying; SHEN Xian-rong; JIANG Shu-qi

doi:10.11653/syfj2013190096

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The Comparison with Kjeldah and Coomassie Brilliant Blue Method on Testing Protein Content of the Polysaccharide from

更新时间：2024-01-04

- The Comparison with Kjeldah and Coomassie Brilliant Blue Method on Testing Protein Content of the Polysaccharide from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 19, Pages: 96-98(2013)
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- DOI：10.11653/syfj2013190096
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- Published：2013
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LIU Yu-ming, QIAN Tian-tian, JIANG Ding-wen, et al. The Comparison with Kjeldah and Coomassie Brilliant Blue Method on Testing Protein Content of the Polysaccharide from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 96-98.
DOI：

LIU Yu-ming, QIAN Tian-tian, JIANG Ding-wen, et al. The Comparison with Kjeldah and Coomassie Brilliant Blue Method on Testing Protein Content of the Polysaccharide from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 96-98. DOI： 10.11653/syfj2013190096.

摘要

目的: 建立一种快速、简单、准确测定方格星虫多糖中蛋白质含量的方法。方法: 分别采用凯氏定氮法和考马斯亮蓝法测定蛋白质含量

并进行稳定性、精密度、重复性和回收率测定。结果: 凯氏定氮法和考马斯亮蓝法测定方格星虫多糖中蛋白的含量分别为2.40%

2.22%

其回收率分别为98.69%

100.97%

2种方法测得蛋白质含量基本相同

无显著性差异(P>0.05)。结论: 综合考虑

凯氏定氮法简便、快速、准确

可用于方格星虫多糖中蛋白质的质量控制。

Abstract

Objective: To establish a simple

rapid and accurate method of protein content analysis of polysaccharide from Sipunculus nudus. Method: Using Kjeldah method and Coomassie brilliant blue method to test protein content of the polysaccharide from S. nudus. Also the precision and accuracy of the two methods were tested. Result: The content of protein was 2.40% and 2.22% by the two method and the average recovery rates were 98.69% and 100.97% respectively. The content by the two methods was almost the same and had no marked difference (P>0.05). Conclusion: In summary

Kjeldah method is simple and accurate and could assay the protein content of polysaccharide from S. nudus rapidly.

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