LI Xian-duan, GU Xue-zhu, CHENG Li-ping, et al. Different Specifications and Different Parts of Quality Evaluation[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 99-104.
DOI:
LI Xian-duan, GU Xue-zhu, CHENG Li-ping, et al. Different Specifications and Different Parts of Quality Evaluation[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 99-104. DOI: 10.11653/syfj2013190099.
Different Specifications and Different Parts of Quality Evaluation
Objective: To evaluate the quality of different specifications and different parts for sale to collect. Method: HPLC method
Kromasil Eternity-5-C18 chromatographic conditions (4.6 mm×250 mm
5 μm)chromatographic column; the mobile phase consisted of acetonitrile (A)-0.4% acetic acid solution (B); gradient elution:0.01 min
15%A
0.01-20 min
20-25%A
35 min
30%A
35-40 min
40%A
50 min
40%A; 50-70 min
15%; volume flow rate of 1 mL·min-1
column temperature was 30℃
the detection wavelength was 280 nm. Result: The Baicalin from the processed strip type Radix scutellariae was 66% higher than the processed Radix scutellariae with xylem rotten. The Baicalin from the pieces of processed strip type Radix scutellariae was 4.06% lower than the pieces of processed Radix scutellariae with xylem rotten. The processed Radix scutellariae with xylem rotten part of almost no Baicalin. The content of baicalin
that below 4 mm Scutellaria baicalensis and fibrous root in about 12%. Conclusion: The processed strip type Radix scutellariae was more expensive than processed Radix scutellariae with xylem rotten. Suggestions of below 4 mm Scutellaria baicalensis and Fibrous root medicinal. The processed Radix scutellariae with xylem rotten part not be medicinal.