Objective: By reversed phase high performance liquid chromatographic method for the simultaneous determination of propolis pinocembrin

chrysin and galangin concentration. Method: YMC-C18 column (4.6 mm×250 mm

5 μm) was used with acetonitrile-0.1% phosphoric acid (40:60) as mobile phase. The flow rate was 1.0 mL·min-1 detected at 268 nm for chrysin and galangin

290 nm for pinocembrin. The column temperature was maintained at 30℃. Result: Pinocembrin

chrysin and galangin were linear respectively in the range of 4.266-426.6

4.59-459

4.322-432.62 mg·L-1. The average recovery rates (n=6) were 98.28%

98.82% and 100.77%; RSD was 0.89%

0.90% and 1.32%. Conclusion: The method is simple

accurate

reproducible

and can be as propolis quality control of Chinese medicinal materials and provide experimental basis.