Objective: To establish the quality standard for Ershiwei Roudoukou pills (Myristicae semen

Aquilariae Lignum Resinatum

Caryophylli Flos and Carthami Flos etc.). Method: Olibanum

Caryophylli Flos

Dalbergiae Odoriferae Lignum

Aquilariae Lignum Resinatum were identified by TLC and HPLC was applied to the quantitative determination of dehydrodiisoeugenol. The method was performed on a Comatex C18 column (4.6 mm×250 mm

5 μm) with methanol-water (68:32) as the mobile phase. UV detection wavelength was set at 274 nm; the column temperature was kept at 35℃ and the flow rate was maintained at 1.0 mL·min-1. Result: The TLC sports were fairly clear

and the blank test showed no interference. Dehydrodiisoeugenol showed a good linear relationship within the range of 0.036 8-0.184 μg (r=0.999 6). The average recovery was 100.25% and RSD was 1.16%. Conclusion: The methods are simple

sensitive

reliable

reproducible

and can be used for the qualitative and quantitative control of Ershiwei Roudoukou pills.