Dynamic Changes of Active Constituents in Different Growth Stage of

ZHANG Lin; LIU Wei; WANG Xiao; DUAN Wen-juan; ZHOU Jie; LIU Feng; ZHANG Qin-de

doi:10.11653/syfj2013190127

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Dynamic Changes of Active Constituents in Different Growth Stage of

更新时间：2024-01-04

- Dynamic Changes of Active Constituents in Different Growth Stage of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 19, Pages: 127-131(2013)
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- DOI：10.11653/syfj2013190127
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- Published：2013
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ZHANG Lin, LIU Wei, WANG Xiao, et al. Dynamic Changes of Active Constituents in Different Growth Stage of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 127-131.
DOI：

ZHANG Lin, LIU Wei, WANG Xiao, et al. Dynamic Changes of Active Constituents in Different Growth Stage of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 127-131. DOI： 10.11653/syfj2013190127.

摘要

目的: 研究不同生育期山东产黄芩各部位活性成分含量的变化。方法: 采用HPLC测定山东莱芜和平阴两地2年生黄芩不同生育期地上和地下部分活性成分的含量。结果: 两地黄芩地上部位的野黄芩苷含量最高

分别为15.46

11.83 mg·g-1。地下部分

莱芜黄芩所含黄芩苷和汉黄芩苷在整个生育期呈下降趋势

最高含量在展叶期

分别为173.60

34.31 mg·g-1

平阴黄芩所含黄芩苷和汉黄芩苷则出现2个最高含量

分别为176.40

212.94 mg·g-1和28.93

34.21 mg·g-1。两地黄芩的黄芩素和汉黄芩素含量分别有2个高峰期

莱芜黄芩所含黄芩素在始花期和采收期达到高峰

分别为8.05

9.49 mg·g-1;汉黄芩素含量分别在始花期和采收期达到高峰

分别为1.94

3.75 mg·g-1。平阴黄芩所含黄芩素和汉黄芩素在展叶期和成熟期达到高峰

分别是12.80

8.27 mg·g-1和2.70

1.48 mg·g-1。结论: 野黄芩苷以地上部位较高

而黄芩苷则以地下部位为高;地下部的黄芩苷和汉黄芩苷含量(从展叶期到采收期)基本呈"M"型变化趋势

黄芩素和汉黄芩素基本呈倒"N"型变化趋势。

Abstract

Objective: To investigate the changes of active composition in different parts and growth stages of Scutellaria baicalensis in Shandong Province. Method: HPLC technology was employed to detect the content of active composition in two-years old plant samples which collected from Laiwu and Pingyin in five different growth stages. Result: The content of scutellarin got to the highest value of 15.46

11.83 mg·g-1 in the part of aboveground in the beginning of flowering stage and leaf-expansion stage

respectively. The content of baicalin and wogonoside in the root which collected from Laiwu decreased in the whole growth stage

the content was 173.60

34.31 mg·g-1respectively

while the content in the samples collected from Pinyin was 176.40

212.94 mg·g-1in flower stage and 28.93

34.21 mg·g-1 in mature stage respectively. The content of baicalein in the sample of Laiwu was 8.05

9.49 mg·g-1in the beginning of flowering stage and maturity stage respectively. The content of wogonin was 1.94

3.75 mg·g-1 in the beginning of flowering stage and withering stage respectively

while the content was 12.80

8.27 mg·g-1and 2.70

1.48 mg·g-1 in leaf-expansion and withering stage in the samples collected from Pingyin. Conclusion: The content of scutellarin in aboveground is higher than that in underground which opposite to the content of baicalin; the content of baicalin and wogonoside from leaf-expansion to withering stage shows a trend of M style

while the content of baicalein and wogonin shows a trend of N style.

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