GC-MS Analysis and GC Determination of Fatty Acids in the Seeds of

SHEN Chang-hui; GAO Jin; WANG Miao; TIAN Chun-lian; LIU Xiao-kun; ZHAO Chun-jie

doi:10.11653/syfj2013190136

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GC-MS Analysis and GC Determination of Fatty Acids in the Seeds of

更新时间：2024-01-04

- GC-MS Analysis and GC Determination of Fatty Acids in the Seeds of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 19, Pages: 136-139(2013)
- 作者机构：
- 作者简介：
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- DOI：10.11653/syfj2013190136
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- Published：2013
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SHEN Chang-hui, GAO Jin, WANG Miao, et al. GC-MS Analysis and GC Determination of Fatty Acids in the Seeds of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 136-139.
DOI：

SHEN Chang-hui, GAO Jin, WANG Miao, et al. GC-MS Analysis and GC Determination of Fatty Acids in the Seeds of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 136-139. DOI： 10.11653/syfj2013190136.

摘要

目的: 分析苘麻子中脂肪酸的组成并测定主要脂肪酸成分的含量。方法: 采用索氏提取法提取其脂肪酸

经甲酯化处理后

用GC-MS联用仪对其脂肪酸组成进行分析和鉴定。采用毛细管气相色谱法

甲酯化后以二十一酸甲酯为内标同时测定苘麻子中棕榈酸、硬脂酸、油酸和亚油酸的含量。结果: 共分离出10种脂肪酸

占脂肪油总量的99.43%。棕榈酸、硬脂酸、油酸和亚油酸的线性分别为33.6~336 mg·L-1(r=0.999 0)

6.58~65.8 mg·L-1 (r=0.999 9)

33.4~334 mg·L-1 (r=0.999 8)

128.3~1 283 mg·L-1 (r=0.999 5)。各成分的平均回收率分别为100.4%

100.6%

101.6%

101.0%

RSD分别为1.3%

1.5%

1.6%

2.1% (n=9)。结论: 鉴定了苘麻子中的10种脂肪酸并首次建立了其中4种主要脂肪酸成分的含量测定方法

可为苘麻子的质量评价提供依据。

Abstract

Objective: To analyze the components of fatty acids in the seeds of Abutilon theophrasti and develop a method for the simultaneous determination of the main fatty acids. Method: Samples were extracted by Soxhlet extraction. After methyl esterification treatment

fatty acids were identified by GC-MS and determined by GC with a FID detector and methyl heneicosanoate as internal standard. Result: Total 10 kinds of fatty acids were identified

accounted for 99.43% of the total fatty oil. The calibration curve was linear within the range of 33.6-336 mg·L-1 (r=0.999 0)

6.58-65.8 mg·L-1 (r=0.999 9)

33.4-334 mg·L-1 (r=0.999 8)

128.3-1 283 mg·L-1 (r=0.999 5) for palmitic acid

octadecanoic acid

oleic acid and linoleic acid respectively. The average recoveries (n=9) were 100.4%

100.6%

101.6% and 101.0% for palmitic acid

octadecanoic acid

oleic acid and linoleic acid respectively. Conclusion: Ten kinds of fatty acids are identified and a method for the determination of palmitic acid

octadecanoic acid

oleic acid and linoleic acid in Abutilon theophrasti simultaneously is developed. The method is sensitive

accurate and reproducible.

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Related Institution

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