Objective: The purpose was to determine the content of ellagic acid and raspberry ketone extracted from red raspberry (Rubus corchorifolius) fruit by HPLC. Method: The chromatography conditions of ellagic acid were as follows:C18 column of ODS CAPCELL PAK (4.6 mm×250 mm

5 μm)

CH3OH-0.2%H3PO4 (45:55) at a flow rate of 1.0 mL·min-1 as mobile phase and detection wavelength of 254 nm; the chromatography conditions of raspberry ketone were as follows:C18 column of ODS CAPCELL PAK (4.6 mm×250 mm

5 μm)

CH3OH-H2O (30:70) at a flow rate of 1.0 mL·min-1 as mobile phase and detection wavelength of 271 nm. Result: The results showed that there was a good linearity between the peak area and the content of ellagic acid in the range of 0.056-0.402 g·L-1 (r=0.999 4). The average recovery of sample was 100.2%

and RSD was 1.5%. The results showed that there was a good linearity between the peak area and the content of raspberry ketone in the range of 0.014 4-0.033 6 g·L-1 (r=0.999 4). The average recovery of sample was 99.4%

and RSD was 1.8%. Conclusion: The method is simple

accurate and reproducible for determination ellagic acid and raspberry ketone in the extract of red raspberry fruit.