Objective: To establish a HPLC fingerprint for the identification of Peach Juvenile Branchlet. Method: The analysis was carried out on a Diamonsil C18(4.6 mm×250 mm

5 μｍ) with a mobile phase consisting of acetonitrile-0.05% phosphoric acid

gradient eluent

the flow rate of 0.5 mL·min-1.The wave length was 290 nm

data were analyzed by the main component of dried branch of Prunus persica and peach leaf and peach seed P.E.to compare the similarity of samples. Result: The mutual mode to HPLC fingerprints was estabilished and the 47 mutual peaks were indicated. The similarities among the of the main component of dried branch of P.persica and peach leaf and peach seed P.E.collected from different sources were obtained. There was an apparent difference in fingerprint. Conclusion: HPLC fingerprint method is repeatable and feasible and can be suitable for identifying Peach Juvenile Branchlet.