Objective: To study the tissue distribution of multiple effective components contained in Qishe pill in rats. Method: The heart

liver

lung

kidney

brain and muscle were collected 30 min

1

2

3

4

6 h

after SD rats were given 0.675 g·kg-1 Qishe pills

then HPLC-MS/MS was used to detect 11 components' concentration in tissues. Result: Flavonoids in Qishe pill was mainly concentrated in brain (calycosin 5.33 μg·g-1

calycosin-7-glucoside 4.94 μg·g-1

formononetin 3.61 μg·g-1

ononin 1.24 μg·g-1) and lung (calycosin 1.38 μg·g-1

calycosin-7-glucoside 15.33 μg·g-1

formononetin 0.29 μg·g-1

ononin 53.75 μg·g-1)

alkaloids was in muscles (sinomenine 347.06 μg·g-1

tetrandrine 706.69 μg·g-1

fangchinoline 589.07 μg·g-1)

terpenes and organic acids were in brain (senkyunolide A 5.75 μg·g-1

senkyunolide I 0.59 μg·g-1

ferulic acid 2.4 0 μg·g-1

cholic acid 56.82 μg·g-1). Conclusion: A variety of active ingredients in Qishe pills has a tendency of the lesion site.