Tissue Distribution of Active Ingredients in Scutellariae Radix from Gegen Qinlian Decoction in Rats

ZHANG Yi-fan; ZHANG Yi-zhu; CHEN Ye; YUAN Jin; AN Rui; WANG Xin-hong

doi:10.11653/syfj2013190177

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Tissue Distribution of Active Ingredients in Scutellariae Radix from Gegen Qinlian Decoction in Rats

更新时间：2024-01-04

- Tissue Distribution of Active Ingredients in Scutellariae Radix from Gegen Qinlian Decoction in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 19, Pages: 177-182(2013)
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- DOI：10.11653/syfj2013190177
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- Published：2013
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ZHANG Yi-fan, ZHANG Yi-zhu, CHEN Ye, et al. Tissue Distribution of Active Ingredients in Scutellariae Radix from Gegen Qinlian Decoction in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 177-182.
DOI：

ZHANG Yi-fan, ZHANG Yi-zhu, CHEN Ye, et al. Tissue Distribution of Active Ingredients in Scutellariae Radix from Gegen Qinlian Decoction in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 177-182. DOI： 10.11653/syfj2013190177.

摘要

目的: 考察大鼠灌服葛根芩连汤后黄芩活性成分在心、肝、胃、肺、脾、肾、小肠中分布特性。方法: 采用LC-MS/MS测定黄芩苷、黄芩素、汉黄芩苷及汉黄芩素在大鼠各组织中的含量

Shiseido CAPCELL PAK C18色谱柱 (2.0 mm×100 mm

5 μm)

流动相0.1%甲酸乙腈-0.1%甲酸水溶液梯度洗脱

采用ESI正离子检测

多反应监测模式测定。按15 g·kg-1灌胃葛根芩连汤

分别于给药后2

12 h断颈动脉放血处死动物

立即分取心、肝、胃、肺、肾、脾、小肠7个组织处理

测定各组织中活性成分的分布情况。结果: 黄芩苷、黄芩素、汉黄芩苷及汉黄芩素的线性范围分别为6.150~3 077

3.030~1 515

6.170~3 085

3.140~1 569 μg·L-1

日内精密度RSD 5.4%~11.7%

日间精密度RSD 7.8%~11.9%

准确度RE-6.4%~7.5%

各组织中4种活性成分的提取回收率均>75.5%

基质效应可忽略

大鼠肝脏组织中4种活性成分在放置4℃保存24 h

-20℃冰箱内反复冻融1

3次及-70℃冰箱内保存4周条件下稳定性良好。结论: 活性成分在各组织中浓度差异较大

大鼠灌服葛根芩连汤后黄芩苷、黄芩素、汉黄芩苷及汉黄芩素主要分布于肝脏、胃及小肠中

其次为肾、心、肺、脾。

Abstract

Objective: To investigate distribution characteristics of active ingredients from Scutellariae Radix in heart

liver

stomach

lung

spleen

kidney

and small intestine after rats being intragastric administrated Gegen Qinlian decoction. Method: LC-MS/MS was employed to determine the contents of baicalin

baicalein

wogonoside and wogonin in rat tissue

Shiseido CAPCELL PAK C18 column(2.0 mm×100 mm

5 μm) with a linear gradient system composed of 0.1% formic acid acetonitrile-0.1% formic acid water solution

ESI under positive ion and multi-reaction monitoring mode(MRM) were adopted.Dose of Gegen Qinlian decoction was 15 g·kg-1

12 h after administration

rats were sacrificed by breaking arteria carotis

then removed heart

liver

stomach

lung

spleen

kidney and small intestine immediately

distribution of active ingredients in each tissue was determined. Result: Linear range of baicalin

baicalein

wogonoside and wogonin were 6.150-3 077

3.030-1 515

6.170-3 085

3.140-1 569 μg·L-1

RSD of the intra was 5.4%-11.7%

RSD of inter-day precision 7.8%-11.9%

RE of accuracy was-6.4%-7.5%

average recoveries of four active ingredients were more than 75.5%

matrix effects could be ignored

the concentration of four active ingredients in liver tissue were stable under different conditions. Conclusion: Active ingredients concentration was quite different in various tissues

major distribution organs of baicalin

baicalein

wogonoside

wogonin in rats were liver

stomach and small intestine

followed by kidney

heart

lung and spleen.

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