LIU Ming-jie, SUN Qi, WANG Qiang, et al. Influence of Total Titerpenoid from var. Preconditioning on Apoptosis of Myocardial Cell in Rabbits Myocardial Ischemia/Reperfusion[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 229-233.
DOI:
LIU Ming-jie, SUN Qi, WANG Qiang, et al. Influence of Total Titerpenoid from var. Preconditioning on Apoptosis of Myocardial Cell in Rabbits Myocardial Ischemia/Reperfusion[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 229-233. DOI: 10.11653/syfj2013190229.
Influence of Total Titerpenoid from var. Preconditioning on Apoptosis of Myocardial Cell in Rabbits Myocardial Ischemia/Reperfusion
Objective: To study the effects of total diterpenoids from Rabdosia japonica var. glaucocalyx (TD) preconditioning on myocardial ischemia/reperfusion injury (MI/RI). Method: Twenty-four rabbits were randomly divided into four groups
sham group (group A)
I/R group(group B)
Danshen dripping pills(DS) group(group C)and TD group (group D). Group A and B were administered distilled water (5 mL·kg-1·d-1) intragastrically daily for 7 days prior to the operation. Rabbits in group C
D underwent the same surgical procedure as those in group B
but received respectively oral DS (37.8 mg·kg-1·d-1) and TD (4.76 mg·kg-1·d-1)
7 days before the operation. Ultrastructure was observed by light microscopy and electron microscopy. TUNEL staining detected myocardial cell apoptosis index. Immunohistochemisty determined expression of apoptosis-related protein B cell lymphoma/leukemia-2(Bcl-2) and Bcl-2 associated x protein(Bax). Result: Apoptosis index of myocardial cells in group C and D is respectively 10.93% and 9.87%
compared with group B (18.40%)the difference was obvious(P<0.05). Immunohistopathological studies showed that A of Bcl-2 in group C
D was 41.03
52.09
compared with group B (29.47) (P<0.05). Conclusion: TD may reduce MI/RI in rabbit model and have protective effects on myocardium.
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