Effect of Alcohol Extractive from Gentianae Macrophyllae Radix on Serum Anti-CCP and TNF- in Collagen Induced Arthritis Rats

WANG Gang; WANG Li-qin; WANG Jia; WANG Tao; LIU Zhuo

doi:10.11653/syfj2013190302

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Effect of Alcohol Extractive from Gentianae Macrophyllae Radix on Serum Anti-CCP and TNF- in Collagen Induced Arthritis Rats

更新时间：2024-01-04

- Effect of Alcohol Extractive from Gentianae Macrophyllae Radix on Serum Anti-CCP and TNF- in Collagen Induced Arthritis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 19, Pages: 302-305(2013)
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- DOI：10.11653/syfj2013190302
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- Published：2013
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WANG Gang, WANG Li-qin, WANG Jia, et al. Effect of Alcohol Extractive from Gentianae Macrophyllae Radix on Serum Anti-CCP and TNF- in Collagen Induced Arthritis Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 302-305.
DOI：

WANG Gang, WANG Li-qin, WANG Jia, et al. Effect of Alcohol Extractive from Gentianae Macrophyllae Radix on Serum Anti-CCP and TNF- in Collagen Induced Arthritis Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(19): 302-305. DOI： 10.11653/syfj2013190302.

摘要

目的: 研究大叶秦艽醇提取物对胶原诱导性关节炎大鼠的防治作用

并初步探讨其作用机制。方法: 随机将96只大鼠分为正常组、模型组、白芍组及秦艽组。除正常组外

余各组用胶原乳液多点皮内注射建立关节炎模型。模型建立后第9天开始秦艽组以秦艽乙醇提物900 mg·kg-1·d-1灌胃

白芍组给予白芍总苷胶囊内容物108 mg·kg-1·d-1灌胃

模型组给予蒸馏水0.5 mL·kg-1·d-1灌胃。造模后14

28 d观察各组大鼠一般情况、关节肿胀程度、ELISA法检测血清抗环瓜氨酸肽(CCP)抗体及肿瘤坏死因子-α(TNF-α)水平。结果: 模型组大鼠的踝关节及足趾关节肿胀明显且血清中抗-CCP抗体及TNF-α水平明显升高

秦艽组及白芍组与模型组比较关节肿胀程度明显减轻

血清中抗-CCP抗体及TNF-α水平明显降低

有统计学意义(P<0.05);免疫后28 d秦艽组与白芍组比较

大鼠关节肿胀程度明显减轻且TNF-α表达降低明显有统计学意义(P<0.05)。结论: 大叶秦艽醇提物能够在早期抑制胶原诱导性关节炎大鼠血清中抗-CCP抗体及TNF-α水平

减轻关节肿胀程度

改善滑膜炎症状

保护关节

体现治疗早期类风湿关节炎(RA)的优势。

Abstract

Objective: To study the effect

of big leaf Gentianae Macrophyllae Radix alcohol extract on serum anti-cyclic citrullinafer peptide(CCP)and tumor necrosis factor-α(TNF-α) in collagen induced arthritis rat control. Method: Ninty-six rats were randomLy divided into:normal group

model group

Paeoniae Alba Radix and Gentianae Macrophyllae Radix group group. In addition to the normal group

each group was given collagen emulsion multipoint to establish arthritis model. After modelling

corresponding drugs was given on day 14

21 and 28

the general status

and joint swelling degree were observed. ELISA method was used to detect serum anti-CCP antibody and TNF-α level. Result: In model rats

ankle and digits joint swelling was obvious and serum anti-CCP antibody and TNF-α level obviously increased

in Gentianae Macrophyllae Radix group and radix paeoniae alba group

compared with model group ankle joint swelling and toes significantly reduced and serum anti-CCP antibody and TNF-α level decreased obviously

there was a statistical significance (P<0.05);after 28 days in Gentianae Macrophyllae Radix group compared with Paeoniae Alba Radix group

joint swelling of rats significantly reduced and TNF-α reduced obviously (P<0.05). Conclusion: The big leaf Gentianae Macrophyllae Radix alcohol extract can decrease in collagen induced arthritis rats serum anti-CCP antibody and TNF-α level

reduce joint swelling degree

and improve the synovial inflammation shape.

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Related Institution

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广州中医药大学脾胃研究所

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