LEI Ling-ling, LIANG Qiao-li, ZOU Nuo-shu, et al. Optimization of Extraction Technology of Active Components from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 1-3.
DOI:
LEI Ling-ling, LIANG Qiao-li, ZOU Nuo-shu, et al. Optimization of Extraction Technology of Active Components from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 1-3. DOI: 10.11653/syfj2013200001.
Optimization of Extraction Technology of Active Components from
Objective: To optimize extracting technology of sparstolonin B and sanleng diphenyllactone from Scirpus yagara. Method: Contents of sparstolonin B and sanleng diphenyllactone were determined by HPLC
chromatographic conditions were as follows:Hanbang column(4.6 mm×250 mm
5 μm)
mobile phase of methanol-1% acetic acid(65:35)
flow rate 1.0 mL·min-1
column temperature 30℃
detection wavelength 385 nm
injection volume 10 μL.With extracting amounts of sparstolonin B and sanleng diphenyllactone as indexes
orthogonal test was adopted to investigate effects of the concentration and dosage of enthanol
extraction time
extraction times on technology. Result: Order of effects of each factors on yield of sparstolonin B was extraction times>ethanol concentration>ethanol consumption>extraction time
but effects of each factor on yield of sanleng diphenyllactone was in order of ethanol concentration>extraction times>extraction time>ethanol consumption.Optimum extraction technology was as following:reflux extracted 2 times with 10 times the amount of 70% ethanol
1.5 h per time;Average extracting amounts of sparstolonin B and sanleng diphenyllactone were 13.95
5.95 μg·g-1
respectively. Conclusion: Optimized extraction technology was stable and feasible with high extraction efficiency;This established determination method was sensitive
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