Objective: To investigate transdermal absorption characteristics of total coumarins in Daphne giraldii and influence of penetration enhancers on transdermal behavior of these ingredients. Method: Valia-Chien diffusion cell was adopted

with the average permeability coefficient

cumulative permeation amounts and skin accumulation amounts of daphnetin as indexes

transdermal absorption kinetics characteristics and effects of penetration enhancer on it were investigated.The content of daphnetin was determined by HPLC

chromatographic conditional were as follows:Kromasil C18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-0.1% phosphoric acid(22:78)

detection wavelength 327 nm

injection volume 20 μL

flow rate 1.0 mL·min-1. Result: Transdermal concentration of daphnetin and time showed a kinetic mode.Absorption time-lag of 1%borneol

3%borneol

5%borneol were 2.3

2.9

3.3 h

respectively.Azone

1

2-propanediol and borneol could obviously promote transdermal absorption of daphnetin

order of the overall penetration effect was azone>borneol>azone and 1

2-propanediol>1

2-propanediol>no penetration enhancer. Conclusion: 3% Azone was the best penetration enhancer for total coumarins in D. giraldii.