Determination of Pulsatilla Saponin D in Pulsatillae Radix from Different Areas

YIN Shan; RAO Xiao-yong; HE Yan; YANG Shi-lin; LUO Xiao-jian

doi:10.11653/syfj2013200036

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Determination of Pulsatilla Saponin D in Pulsatillae Radix from Different Areas

更新时间：2024-01-04

- Determination of Pulsatilla Saponin D in Pulsatillae Radix from Different Areas
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 20, Pages: 36-38(2013)
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- DOI：10.11653/syfj2013200036
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- Published：2013
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YIN Shan, RAO Xiao-yong, HE Yan, et al. Determination of Pulsatilla Saponin D in Pulsatillae Radix from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 36-38.
DOI：

YIN Shan, RAO Xiao-yong, HE Yan, et al. Determination of Pulsatilla Saponin D in Pulsatillae Radix from Different Areas[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 36-38. DOI： 10.11653/syfj2013200036.

摘要

目的: 建立白头翁中白头翁皂苷D的含量测定方法。方法: 采用HPLC测定白头翁皂苷D的含量

色谱条件为依利特Hypersil ODS2 C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水-甲酸(38:62:0.1)

流速1.0 mL·min-1

检测波长203 nm

柱温25℃

考察不同产地白头翁中白头翁皂苷D的含量差异。结果: 白头翁皂苷D线性范围0.311 2~3.112 μg(r=0.999 9)

精密度RSD 1.36%

重复性RSD 1.65%

供试品溶液在24 h内稳定

平均加样回收率98.07%(RSD 0.64%)。吉林产白头翁中白头翁皂苷D含量最高(0.256%)

比广东产白头翁高了约20倍。结论: 该测定方法简便易行、结果准确、重复性好。不同产地的白头翁中白头翁皂苷D的含量差异较大

选用时应慎重。

Abstract

Objective: To establish a determination method for pulsatilla saponin D in Pulsatillae Radix. Method: The content of pulsatilla saponin D was determined by RP-HPLC

chromatographic conditions were as follows:Elite Hypersil ODS2 C18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-water-formic acid(38:62:0.1)

flow rate 1.0 mL·min-1

detection wavelength 203 nm

column temperature 25℃.Content difference of pulsatilla saponin D in Pulsatillae Radix from different areas was investigated. Result: Pulsatilla saponin D had good linear relationship in 0.311 2-3.112 μg(r=0.999 9)

RSD of instrument precision was 1.36%

RSD of method repeatability was 1.65%

samples solution was stable within 24 h

the average recovery was 98.07%(RSD 0.64%).The content of pulsatilla saponin D in Pulsatillae Radix from Jilin was the highest(0.256%)

which was 20 folds higher than Pulsatillae Radix from Guangdong. Conclusion: This method was simple

accurate and repeatable.The content of pulsatilla saponin D in Pulsatillae Radix from different areas had much difference

so materials choice should be careful.

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